Molecular Biology-based Outcomes
These are for ELISA, Western Blot and qRT-PCR analyses of the brain/spinal cord tissue samples to determine the relative expression of multiple inflammatory, neurotrophic and other cytokines.
- BioTek multi-Mode Synergy LX Microplate Reader for common endpoint assays including nucleic acid and protein quantification, ELISA, BSA, Bradford and cell viability.
- Protein A/G agarose beads (Santa Cruz Biotechnology, USA) for immunoprecipitation (IP)
- QIA cube fully automated DNA, RNA, and Protein purification system (Qiagen, USA)
- Ultrasound Sonicator (Qsonica, USA), for chromatin immunoprecipitation (ChIP)
- LightShift Chemiluminescent EMSA Kit (Thermoscientific, USA)
- TransBlot system with Gel documentation (BioRad, USA)
- DNA agarose gel electrophoresis system (BioRad, USA)
- Tissue Lyzer (Qiazen, USA) for western blot (WB), etc.
- iBlotTM gel transfer system for WB (Invitrogen, USA)
- Nanodrop Spectrophotometer (DeNovix, USA)
- C6 Flow Cytometer (Accuri, USA)
- qRT-PCR system (BioRad, USA)