Molecular Biology

Molecular Biology-based Outcomes 

These are for ELISA, Western Blot and qRT-PCR analyses of the brain/spinal cord tissue samples to determine the relative expression of multiple inflammatory, neurotrophic and other cytokines.

  • BioTek multi-Mode Synergy LX Microplate Reader for common endpoint assays including nucleic acid and protein quantification, ELISA, BSA, Bradford and cell viability.
  • Protein A/G agarose beads (Santa Cruz Biotechnology, USA) for immunoprecipitation (IP)
  • QIA cube fully automated DNA, RNA, and Protein purification system (Qiagen, USA)
  • Ultrasound Sonicator (Qsonica, USA), for chromatin immunoprecipitation (ChIP)
  • LightShift Chemiluminescent EMSA Kit (Thermoscientific, USA)
  • TransBlot system with Gel documentation (BioRad, USA)
  • DNA agarose gel electrophoresis system (BioRad, USA)
  • Tissue Lyzer (Qiazen, USA) for western blot (WB), etc.
  • iBlotTM¬†gel transfer system for WB (Invitrogen, USA)
  • Nanodrop Spectrophotometer (DeNovix, USA)
  • C6 Flow Cytometer (Accuri, USA)
  • qRT-PCR system (BioRad, USA)